Residual Ret signal was detected in the C4da neuron soma in Ret mutant animals but not in dendrites ( E inset). ( D) Ret was also present in distal terminal dendrites of the dorsal field of C4da neurons ( D′ and D′′, scale bar 20 μm, 10 μm for insets). Resliced portions (in Z direction) of primary ( C′) and terminal ( C′′) C4da dendrites show that Ret strongly labels the basal side of dendrites facing the ECM as shown in the schematic drawing. Overlays with GFP expressing C4da neurons ( ppk-Gal4 > CD4-tdGFP) show that specific anti-Ret signal could be detected throughout the C4da dendritic arbor ( C). ( C– E) Immunohistochemical analysis of Ret expression in third instar larvae of wildtype ( C and D) and Ret deficient animals ( E). ( B) A Gal4 insertion in the Ret genomic locus drives CD8-GFP expression in C4da neurons indicating the presence of Ret. Confocal live images of control animals (ctl) show wildtype dendrite morphology, while expression of either of two independent Ret-RNAi transgenes led to severely disorganized dendrites with incomplete receptive field coverage. ( A) Ret RNAi transgenes together with Dcr2 were driven by Gal4 21–7 and C4da neuron morphology was visualized with a specific fluorescent reporter ( ppk-CD4-tdTomato). melanogaster cell adhesion cell-matrix interactions dendrite development extracellular matrix neuroscience patterning receptors. We propose novel functions for Ret as a regulator in dendrite patterning and adhesion distinct from its role in axon guidance.ĭ. In addition, Ret is required for dendrite stability and normal F-actin distribution suggesting it has an essential role in dendrite maintenance. We provide evidence that Ret interacts with integrins to regulate dendrite adhesion via rac1. The loss of Ret results in severe dendrite defects due to loss of extracellular matrix adhesion, thus impairing growth within a 2D plane. We conducted an in vivo RNAi screen in Drosophila class IV dendritic arborization (C4da) neurons and identified the conserved Ret receptor, known to play a role in axon guidance, as an important regulator of dendrite development. Although cell surface cues play a major role in this process, few dendrite specific signals have been identified to date. Neurons develop highly stereotyped receptive fields by coordinated growth of their dendrites.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |